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How To Make A Blood Smear. This drop of blood is then placed on a clean glass slide about 1 cm from one end in the center line of the slide. 3 Place the end of the spreader slide on the sample slide so that the short sided edge of the spreader is below the drop of blood. Drop of blood too large or too small 2. Otherwise your smear may run right off of your horizontal slide and you will not have a nice feathered edge and your smear will be so thin you will have trouble finding enough cells.
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Clean slides with 70 to 90 alcohol and allow to dry. Place a small drop of blood or one side about 1-2 cm from one end. Aim for a blood droplet size of 4mm diameter. This PULLS the blood across to make the smear. A quick instructional video demonstrating. Common cause of a poor blood smear.
Put a small size of blood drop on each glass slide about half centimeter from its narrow edge on the right side.
The process is similar to making a normal thick film but instead of using a stick to spread the blood the edge of a glass microscope slide is used while applying firm pressure to create small scratches in the underlying slide. The main part of the blood film. Place either the long or the short edge of the pusher slide just in front of the blood drop holding it at an approximately 30-degree angle from the patient slide. Without delay place a spreader at an angle of 45 from the slide and move it back to make contact with the drop. Clean the area to be punctured with 70 alcohol. Aim for a blood droplet size of 4mm diameter.
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3 Place the end of the spreader slide on the sample slide so that the short sided edge of the spreader is below the drop of blood. This PULLS the blood across to make the smear. Select the finger to puncture usually the middle or ring finger. Take one drop of venous or capillary blood. Blood can also be taken from an EDTA sample.
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3 Place the end of the spreader slide on the sample slide so that the short sided edge of the spreader is below the drop of blood. Optimise spreading speed for length and a good feathered edge. The feathered edge the monolayer and the body and base of the smear. The blood smear is a simple blood test. Do not touch the surface of the slide where the blood smear will be made.
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Blood can also be taken from an EDTA sample. Do not touch the surface of the slide where the blood smear will be made. A quick instructional video demonstrating. Collect blood in an EDTA tube and make the smears when back at the clinic. Take one drop of venous or capillary blood.
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This PULLS the blood across to make the smear. Specimen of blood can also be taken by venepuncture. Making a quality blood smear Although there are several techniques described for making blood smears most people use the wedge or push technique. Do not touch the surface of the slide where the blood smear will be made. To make a short smear hold the spreader at a steeper angle and to make a longer smear hold it closer to the drop.
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Blood Smear Technique 1 On one slide the sample slide place a small drop of blood 2 Use the second slide as a spreader slide. This drop of blood is then placed on a clean glass slide about 1 cm from one end in the center line of the slide. 3 Place the end of the spreader slide on the sample slide so that the short sided edge of the spreader is below the drop of blood. Place either the long or the short edge of the pusher slide just in front of the blood drop holding it at an approximately 30-degree angle from the patient slide. Blood can also be taken from an EDTA sample.
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Proper preparation of the blood smear is critical for correct interpretation and obtaining accurate results. Prick the ring finger of left hand with the help of prickling needle under all aseptic conditions discard the first two drOps of blood from skin puncture site. To make a short smear hold the spreader at a steeper angle and to make a longer smear hold it closer to the drop. The tapering end of the blood film. In this video I quickly go over how to make a peripheral blood smear.
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Making a quality blood smear Although there are several techniques described for making blood smears most people use the wedge or push technique. The feathered edge the monolayer and the body and base of the smear. 3 Place the end of the spreader slide on the sample slide so that the short sided edge of the spreader is below the drop of blood. I hope you can see the smear well enough in the video if not please leave a comment and. Blood Smear Technique 1 On one slide the sample slide place a small drop of blood 2 Use the second slide as a spreader slide.
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How to perform a blood film in 3 easy stepsLearn more and visit httpswwwidexxcouk. All parts of the smear should be examined however the monolayer is the area where the cells are examined in close detail and differential cell. Clean the area to be punctured with 70 alcohol. Use clean high-quality microscope slides. Components of a blood smear.
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Always start with room temperature well-mixed clot free EDTA anticoagulated blood heparin is used for some exotic species. Push the edge of the pusher slide into the blood drop so that it spreads out completely along the pusher slide. The drop and it spreads by capillary action along its edge. Making a quality blood smear Although there are several techniques described for making blood smears most people use the wedge or push technique. Blood can also be taken from an EDTA sample.
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Preparation of blood smear 1 Thin Blood Film Thin PBF can be prepared from anticoagulated blood obtained by venipuncture or from free-flowing finger-prick blood by any of the following three techniques. Place a small drop of blood or one side about 1-2 cm from one end. The drop and it spreads by capillary action along its edge. All parts of the smear should be examined however the monolayer is the area where the cells are examined in close detail and differential cell. Take another clean slide and holding at an angle of about 45 deg touch the blood with one end of the slide so the blood runs along the edge of the slide by capillary action.
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Proper preparation of the blood smear is critical for correct interpretation and obtaining accurate results. A phlebotomist a person specifically trained to draw blood first cleans and sterilizes the injection site with an antiseptic. Blood can also be taken from an EDTA sample. In infants puncture the heel. Making a quality blood smear Although there are several techniques described for making blood smears most people use the wedge or push technique.
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A quick instructional video demonstrating. Collect blood in an EDTA tube and make the smears when back at the clinic. Do not touch the surface of the slide where the blood smear will be made. Prick the ring finger of left hand with the help of prickling needle under all aseptic conditions discard the first two drOps of blood from skin puncture site. Place a drop of blood on the patient slide near the frosted edge.
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Blood can also be taken from an EDTA sample. The slide should be clean. Proper preparation of the blood smear is critical for correct interpretation and obtaining accurate results. If the blood has been refrigerated allow it to return. The drop and it spreads by capillary action along its edge.
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Making a quality blood smear Although there are several techniques described for making blood smears most people use the wedge or push technique. Without delay place a spreader at an angle of 45 from the slide and move it back to make contact with the drop. The scratches allow for improved adherence of the blood film to the slide without affecting the smear morphology. The slide should be clean. Put a small size of blood drop on each glass slide about half centimeter from its narrow edge on the right side.
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Do not push the blood by having it ahead of the smearing slide. The portion of blood film near the drop of blood. A quick instructional video demonstrating. Optimise spreading speed for length and a good feathered edge. Use clean high-quality microscope slides.
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Collect blood in an EDTA tube and make the smears when back at the clinic. Push the edge of the pusher slide into the blood drop so that it spreads out completely along the pusher slide. The drop and it spreads by capillary action along its edge. In infants puncture the heel. Place either the long or the short edge of the pusher slide just in front of the blood drop holding it at an approximately 30-degree angle from the patient slide.
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Drop of blood too large or too small 2. They then tie a band above. Take one drop of venous or capillary blood. Otherwise your smear may run right off of your horizontal slide and you will not have a nice feathered edge and your smear will be so thin you will have trouble finding enough cells. The slide should be clean.
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The feathered edge the monolayer and the body and base of the smear. Specimen of blood can also be taken by venepuncture. Use clean high-quality microscope slides. Push carefully along the length of the first slide to produce a thin smear of blood. Clean the area to be punctured with 70 alcohol.
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